Vimentin molecular weight sigma

2019-12-14 18:47

See product citations (96) Vimentin Antibody (RV202) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 gml. raised against cytoskeletal Vimentin extracts of calf lens. recommended for detection of Vimentin of mouse, rat and human origin by WB, IP, IF, IHC(P) and FCM; also reactive with additional species, including bovine.Ab recognizes full length recombinant Human vimentin (ab ) which has an expected molecular weight of 54 kDa. vimentin molecular weight sigma

Lyse cells by adding 1X SDS sample buffer (100 l per well of 6well plate or 500 l for a 10 cm diameter plate). Immediately scrape the cells off the plate and transfer the extract to a microcentrifuge tube. Keep on ice. Sonicate for 1015 sec to complete cell lysis and shear DNA (to reduce sample viscosity).

Western blot analysis of whole cell lysates probed with vimentin antibody (VMA ) followed by detection with HRP conjugated Goat anti Human IgG F(ab') 2 (, STAR126P) and visualized on the ChemiDoc MP with 2 second exposure. Arrow points to vimentin (molecular weight 54 kDa). This AntiVimentin Antibody, clone 43BE8 is validated for use in IC, IH(P) for the detection of Vimentin. SigmaAldrich vimentin molecular weight sigma Recognizes vimentin in cultured mammalian cells and tissue sections. AB1620 specifically stains the vimentin band (58 kD) using total cell or tissue extracts in immunoblotting. No reactivity to desmin, keratin, neurofilaments or glial filaments.

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